Journal: Oxidative Medicine and Cellular Longevity

Article Title: Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2

doi: 10.1155/2022/1792412

Figure Lengend Snippet: Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. n = 8. (e) HE histological scores of mouse NP tissues by HE staining. n = 8. ∗ p < 0.05 vs. mice without any injection; # p < 0.05 vs. IVDD mice injected with harboring shRNA against shRNA NC; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring overexpression vector NC; @ p < 0.05 vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. n = 8.

Article Snippet: Afterwards, plasmids of sh-NC, sh-GAS5, oe-NC, oe-GAS5, miR-17-3p mimic, and miR-17-3p inhibitor were packaged into lentivirus vectors (pSIH1-H1-copGFP and pLV-EGFP-N) by GenePharma Co., Ltd. (Shanghai, China) to generate lentiviruses including pSIH1-H1-copGFP-sh-NC, pSIH1-H1-copGFP-sh-GAS5, pLV-EGFP-NC, pLV-EGFP-GAS5, pSIH1-H1-copGFP-miR-17-3p inhibitor, and pLV-EGFP-miR-17-3p, respectively.

Techniques: In Vivo, Inhibition, Western Blot, TUNEL Assay, Expressing, Staining, Injection, shRNA, Over Expression, Plasmid Preparation, Standard Deviation

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: lncRNA-ORLNC1 Is Associated with Osteoporosis and Differentiation of BMSCs

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques:

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: lncRNA-ORLNC1 Inhibits Osteogenesis and Stimulates Adipogenesis of BMSCs

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques:

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: Overexpression of lncRNA-ORLNC1 Results in Osteoporosis and Impairs Osteogenic Capacity in a Mouse Model of Osteoporosis

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques: Over Expression

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: Knockdown of lncRNA-ORLNC1 Increases Osteoblast Maturation and Decreases Adipogenesis of BMSCs

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques:

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: lncRNA-ORLNC1 Directly Targets miR-296, and miR-296 Possesses the Ability to Regulate Osteogenic Differentiation of BMSCs

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques:

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: lncRNA-ORLNC1 Regulates Osteogenesis and Adipogenesis of BMSCs through Targeting miR-296, and Pten Is a Candidate Target Gene for miR-296

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques:

Journal: Molecular Therapy

Article Title: The Long Non-coding RNA-ORLNC1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Fate

doi: 10.1016/j.ymthe.2018.11.019

Figure Lengend Snippet: The Sequences of Primers

Article Snippet: The packaged lncRNA-ORLNC1-overexpressing lentivirus vector and NC lentivirus vector were obtained from Cyagen Biosciences.

Techniques: